Part:BBa_K4728009

TpiB (Transmembrane Protein)

Originating from Comamonas sp. E6, TpiB is a part of the TpiA-TpiB transmembrane proteins. TpiB is constitutively expressed as a single operon in E6 cells TpiB representing the small transmembrane protein. Together alongside TpiA, the TpiA-TpiB membrane components interact in conjunction with TphC for encoding a tripartite transporter required for TPA uptake, and involved in uptake of IPA, OPA, PCA, and citrate. The TpiA-TpiB system is dependent on the proton motive force, thus inhibitors of the protonophore result in complete inhibition of TPA uptake (Hosaka et al., 2013).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 451
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 451
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 451
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 451
  Illegal NgoMIV site found at 291
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Hosaka, M., Kamimura, N., Toribami, S., Mori, K., Kasai, D., Fukuda, M., and Masai, E. (2013). Novel Tripartite Aromatic Acid Transporter Essential for Terephthalate Uptake in Comamonas SP. strain E6. Applied and Environmental Microbiology, 79(19), 6148-6155. https://doi.org/10.1128/AEM.01600-13