Coding

Part:BBa_K4728006

Designed by: Michelle Li   Group: iGEM23_Queens-Canada   (2023-10-08)


TphA1 (Reductase)

TphA1 encodes for the reductase component of TPADO. This component works in conjunction with the oxigenase component (TphA2 and TphA3) and tphB in order to facilitate TPA degradation (Sasoh et al., 2006).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 433
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 433
    Illegal AgeI site found at 366
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Sasoh, M., Masai, E., Ishibashi, S., Hara, H., Kamimura, N., Miyauchi, K., & Fukuda, M. (2006). Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Applied and environmental microbiology, 72(3), 1825–1832. https://doi.org/10.1128/AEM.72.3.1825-1832.2006

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