Regulatory

Part:BBa_K4728000

Designed by: Bryant Han   Group: iGEM23_Queens-Canada   (2023-09-05)


TPA-inducible promoter

This part is the sequence of the TPA-induced promoter (P_tph) originally from Comamonas testosteroni. The promoter is activated by tphR, the activator protein of P_tph, which is activated by TPA-binding. In other words, when tphR binds to TPA, it will in turn activate P_tph.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 80
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Kasai, D., Kitajima, M., Fukuda, M., & Masai, E. (2010). Transcriptional regulation of the terephthalate catabolism operon in Comamonas sp. strain E6. Applied and environmental microbiology, 76(18), 6047–6055. https://doi.org/10.1128/AEM.00742-10

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