Part:BBa_K4727107

sgDNA for OmpA125 in K. pneumoniae

This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae. The guide binds 125bp downstream the TSS, on the RV strand of the gene.

OmpA is a predominant outer membrane protein in Enterobacteriaceae. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In Klebsiella pneumoniae, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer[1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Bosshart, P. D., Iordanov, I., Garzon-Coral, C., Demange, P., Engel, A., Milon, A., & Müller, D. J. (2012). The Transmembrane Protein KpOmpA Anchoring the Outer Membrane of Klebsiella pneumoniae Unfolds and Refolds in Response to Tensile Load. Structure, 20(1), 121–127.