DNA

Part:BBa_K4727105

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-09-15)


sgDNA for BlaSHV-1 85 in K. pneumoniae

This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae . The guide binds 85bp downstream the TSS.

The penicillin resistance gene blaSHV-1 was first discovered in K. pneumoniae in the 1960s. It is a class A beta-lactamase and also has β-lactamase activity against cephalosporins. It determines resistance against ampicillin. Over the years, many genes for broad-spectrum β-lactamases have developed, and have been identified, in various clinical strains of K. pneumoniae, increasing the emergence of infections caused by this bacterium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None