Part:BBa_K4727101

sgDNA for Orf3/CraA 30 for A. baumannii

This guide DNA has been designed to work with dCas9 to target AMR related genes in Acinetobacter baumannii . The guide binds 30bp downstream the TSS

CraA (which stands for chloramphenicol resistance Acinetobacter, originally named Orf3) is a multidrug efflux transport protein homologous to the MdfA efflux pump of Escherichia coli. The system has been found in all 82 A. baumannii strains tested in [1] and is believed to contribute to intrinsic resistance to chloramphenicol [1]. The silencing of this gene significantly decreases the MIC (Minimum Inhibitory Concentration) for the antibiotic chloramphenicol [2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Coyne, Sébastien, Patrice Courvalin, e Bruno Périchon. «Efflux-Mediated Antibiotic Resistance in Acinetobacter Spp». Antimicrobial Agents and Chemotherapy 55, fasc. 3 (marzo 2011): 947–53. https://doi.org/10.1128/AAC.01388-10.

[2] Roca, I., S. Marti, P. Espinal, P. Martínez, I. Gibert, e J. Vila. «CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter Baumannii». Antimicrobial Agents and Chemotherapy 53, fasc. 9 (settembre 2009): 4013–14. https://doi.org/10.1128/AAC.00584-09.