Plasmid_Backbone

Part:BBa_K4727001

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-10-02)


pPAC plasmid backbone for P. aeruginosa

This part is a new standard backbone intended to be used for cloning different types of genes in P. aeruginosa. It is particularly suitable for PAO1 strain, thanks to its chloramphenicol marker of resistance. Indeed PAO1 is resistant to both kanamycin and ampicillin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal suffix found at 1007
    Illegal EcoRI site found at 987
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 987
    Illegal SpeI site found at 1008
    Illegal PstI site found at 1022
    Illegal NotI site found at 993
    Illegal NotI site found at 1015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 987
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 1008
    Illegal EcoRI site found at 987
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 987
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1008
    Illegal PstI site found at 1022
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1198
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None