Coding
Part:BBa_K4724002
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-06)
IsPETase
IsPETase is a polyethylene terephthalate degrading enzyme first identified in Ideonella sakaiensis. IsPETase can decompose PET and release soluble products such as MHET, and TPA. IsPETase's ability to degrade PET is limited by its low stability. The crystallinity of the substrate also affects the function of IsPETase in degrading plastics, and IsPETase degrades less effectively in PET samples with higher crystallinity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |