Device

Part:BBa_K4720021

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


J23100+RBS+FleQ+TT+Pcl+Ppel+RBS+GFP+T

BBa_J23100 + RBS (BBa_J34801) FleQ (BBa_K4720000) + Double Terminator (BBa_B0015) + Pcl (BBa_R0051) + Ppel (BBa_K4242001) + RBS (BBa_J34801) + GFP (BBa_E0040) + Terminator (BBa_B1006)

This part is our adaptation of the BBa_K4242013 part by CUG China 2022. Improvements that were made in this design are the removal of repeating sequences larger than 29 bp (RBS + scars). This was done after first hand experience of recombination with the repeating Pcl sequence (see BBa_K4720017) and the repeating double terminator sequence (see BBa_K4720015).

This part is the combination of parts BBa_K4720017 and BBa_K4720012. Because of problems with obtaining part BBa_K4720017, due to recombination, we were unable to obtain the this part and perform subsequent experiments with it. We do leave our design here for future iGEM teams to use.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2553


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Categories
Parameters
None