Composite

Part:BBa_K4720016

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


Ptac+RBS+GFP+TT+Pcl+RBS+RFP+TT

Ptac (BBa_K180000) + RBS (BBa_J34801) + GFP (BBa_E0040) + Double Terminator (BBa_B0015) + Pcl (BBa_R0051) + RBS (BBa_B0034) + RFP (BBa_E1010) + Double Terminator (BBa_B0015).

The aim of this construct was to test how much the GFP and RFP reporters intervene with each other. There is small overlap in emission and exploitation spectra. The magnitude would be tested by using different IPTG concentrations and comparing the results to the controls BBa_K4720019 and BBa_K4720024. The construct was however not obtained due to recombination with the repeating double terminator sequence when the parts BBa_K180000 and BBa_K4720015 were combined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2968
    Illegal AgeI site found at 3080
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2117


[edit]
Categories
Parameters
None