Reporter

Part:BBa_K4720015

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


RBS+GFP+TT+Pcl+RBS+RFP+TT

RBS (BBa_J34801) + GFP (BBa_E0040) + Double Terminator (BBa_B0015) + Pcl (BBa_R0051) + RBS (BBa_B0034) + RFP (BBa_E1010) + Double Terminator (BBa_B0015).

This part is the original design we had for the complete cyclic-di-GMP reporter sequence + the RFP reporter sequence that was planed to be used for normalization of the previous. This part was successfully obtained, but in subsequent cloning steps we encountered problems with recombination, due to the repeating sequence of the double terminator. This would result in the subsequent loss of the RFP reporter part of the construct. The GFP reporter part of the construct was redesigned with a unique terminator sequence (BBa_K4720012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1513
    Illegal AgeI site found at 1625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


[edit]
Categories
Parameters
None