Primer

Part:BBa_K4717003

Designed by: Jun Zhou   Group: iGEM23_BIBS-beijing-china   (2023-10-11)


HK1 primer

In this project, we used Primer3 (https://bioinfo.ut.ee/primer3-0.4.0/) to design the primer sequence based on information of NCBI nucleoside database. We designed three primers for each gene and selected one with the best specificity and efficiency. The designed primary was then synthesized by solid-phase oligonucleotide synthesis method using an automated DNA synthesizer.

Primer 1:

OLIGO start len tm gc% any 3' rep seq
LEFT 17201 20 59.99 45.00 6.00 0.00 AGGCCAACTCTGCAAAGAAA
RIGHT 17445 20 69.94 40.00 6.00 0.00 GCACCTGGCCAAAATAAAAA

SEQUENCE SIZE: 18021 INCLUDED REGION SIZE: 18021 PRODUCT SIZE: 245, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

Primer 2:

OLIGO start len tm gc% any 3' seq
LEFT 1897 20 60.05 55.00 2.00 2.00 CTGGCTGTGTCCTTGCTGTA
RIGHT 2055 20 59.95 50.00 3.00 0.00 CCCATACCTTAGCGTGGAAA

PRODUCT SIZE: 159, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

Primer 3:

OLIGO start len tm gc% any 3' seq
LEFT 1898 20 60.05 55.00 2.00 2.00 TGGCTGTGTCCTTGCTGTAG
RIGHT 2055 20 59.95 50.00 3.00 0.00 CCCATACCTTAGCGTGGAAA



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None