Primer
Part:BBa_K4717002
Designed by: Jun Zhou Group: iGEM23_BIBS-beijing-china (2023-10-10)
LDHA primer
In this project, we used Primer3 (https://bioinfo.ut.ee/primer3-0.4.0/) to design the primer sequence based on information of NCBI nucleoside database. We designed three primers for each gene and selected one with the best specificity and efficiency. The designed primary was then synthesized by solid-phase oligonucleotide synthesis method using an automated DNA synthesizer.
Primer 1:
| OLIGO | start | len | tm | gc% | any | 3' | rep | seq |
|---|---|---|---|---|---|---|---|---|
| LEFT | 20178 | 20 | 59.99 | 60.00 | 6.00 | 0.00 | 11.00 | CCTGAGCCCAGAACACTCTC |
| RIGHT | 20335 | 20 | 60.00 | 55.00 | 4.00 | 2.00 | 11.00 | GTGGAAGCAACTCCTTGAGC |
SEQUENCE SIZE: 131883 INCLUDED REGION SIZE: 131883 PRODUCT SIZE: 158, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
Primer 2：
| OLIGO | start | len | tm | gc% | any | 3' seq |
|---|---|---|---|---|---|---|
| LEFT | 130138 | 20 | 60.01 | 55.00 | 7.00 | AGCCACTGGCAGGACTAGAA |
| RIGHT | 130334 | 20 | 59.99 | 50.00 | 4.00 | GCTGAAGATGCTTGTGGTGA |
PRODUCT SIZE: 197, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
Primer 3：
| OLIGO | start | len | tm | gc% | any | 3' seq |
|---|---|---|---|---|---|---|
| LEFT | 105214 | 20 | 59.99 | 55.00 | 4.00 | ACACATAGGAAGGGCCACAG |
| RIGHT | 105420 | 20 | 60.01 | 55.00 | 3.00 | AGCCTCTGTGTAGCCCTGAA |
PRODUCT SIZE: 207, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| None |