Part:BBa_K4717001
GLUT1 primer
In this project, we used Primer3 (https://bioinfo.ut.ee/primer3-0.4.0/) to design the primer sequence based on information of NCBI nucleoside database. We designed three primers for each gene and selected one with the best specificity and efficiency. The designed primary was then synthesized by solid-phase oligonucleotide synthesis method using an automated DNA synthesizer.
the sequence of three primer was showed as following：
Primer 1：
| OLIGO | start | len | tm | gc% | any | 3' rep | seq |
|---|---|---|---|---|---|---|---|
| LEFT | 12244 | 20 | 59.99 | 55.00 | 3.00 | 5.00 | TCCCACTTGGCAGACTCTCT |
| RIGHT | 12486 | 20 | 60.01 | 60.00 | 3.00 | 8.00 | ACTGGACCCAGTCCTGACAC |
SEQUENCE SIZE: 33516 INCLUDED REGION SIZE: 33516 PRODUCT SIZE: 243, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR NC_000001.11:c42958868-42925353 Homo sapiens chromosome 1, GRCh38.p14 Primary Assembly
Primer 2：
| OLIGO | start | len | tm | gc% | any | 3' seq |
|---|---|---|---|---|---|---|
| LEFT | 12319 | 20 | 59.97 | 55.00 | 4.00 | GTACCCAGGCAAAACTGGAA |
| RIGHT | 12486 | 20 | 60.01 | 60.00 | 8.00 | ACTGGACCCAGTCCTGACAC |
PRODUCT SIZE: 168, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
Primer 3：
| OLIGO | start | len | tm | gc% | any | 3' seq |
|---|---|---|---|---|---|---|
| LEFT | 12319 | 20 | 59.97 | 55.00 | 4.00 | GTACCCAGGCAAAACTGGAA |
| RIGHT | 12486 | 20 | 60.01 | 60.00 | 8.00 | ACTGGACCCAGTCCTGACAC |
PRODUCT SIZE: 155, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |