Part:BBa_K4716991
PET-NorR-ProA-PnorV-pep-his tag
On the direction for our PnorV regulator gene, there is a constitutive ProA promoter (BBa_K4716999) induce the expression of NorR protein (BBa_K4716998); On the opposite direction for our gene expression are the PnorV promoter which can bind with NorR protein when there is no nitric oxygen in environment, RBS (BBa_B0034) which is the ribosome binding site, our target gene NSP4-pep, 6-his tag as our antibody binding site in our protein analysis process and the double terminator (BBa_B0015) is the terminator. On our backbone pET, the resistant gene of ampicilin has the function for screening the successful transformed bacteria. Near the gene of Rop protein there is also an pBR332 replication origin for the whole vector.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3792
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3038
Illegal XhoI site found at 3801 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2027
Illegal AgeI site found at 1030 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 61
Illegal BsaI.rc site found at 3159
Illegal SapI site found at 1254
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