Plasmid

Part:BBa_K4716990

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


pET-J23119-fvp-sfGFP

On the direction for our target gene we use the constitutive promoter J23119 (BBa_J23119), RBS (BBa_B0034) is the ribosome binding site, our target gene NSP4-Fvp, the sfGFP is the reporter gene and the double terminator (BBa_B0015) is the terminater we use in this circuit. On our backbone pET, the resistant gene of ampicilin has the function for screening the successful transformed bacteria. Near the gene of Rop protein there is also an pBR332 replication origin for the whole vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
    Illegal AgeI site found at 481
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 139
    Illegal BsaI site found at 484
    Illegal BsaI site found at 751
    Illegal SapI site found at 640
    Illegal SapI.rc site found at 1725


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Categories
Parameters
None