Plasmid

Part:BBa_K4716988

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


pET-J23119-sfGFP

As the control group plasmid in enzymatic release module, on the direction for our target gene we use the constitutive promoter J23119 (BBa_J23119), RBS (BBa_B0034) is the ribosome binding site, sfGFP gene is our fluorescent reporter gene, double terminator (BBa_B0015) is the terminator. On our backbone pET, the resistant gene of ampicilin has the function for screening the successful transformed bacteria. Near the gene of Rop protein there is also an pBR332 replication origin for the whole vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 73


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