Coding

Part:BBa_K4716987

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


Fvp-p-6his tag

Fvp-p is a prolyl endopeptidase gluten breaking enzyme from Enokitake (Flammulina velutipes), which can break down the 33-mer toxic peptide of gluten for celiac disease patients that can not be completely broken down in the intestine more efficiently. We sequence it to be expressed in E. coli and secrete the enzyme outside the gut microbiota. And for this part we design it for adapt the plasmid we want it to express under the control of NO sensor, so we add a his-tag at the end of Fvp-p.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10
    Illegal BsaI site found at 355
    Illegal BsaI site found at 622
    Illegal SapI site found at 511


[edit]
Categories
Parameters
None