Part:BBa_K4716010

pSB1C3-J23119-CsgA-cp19k-mSandy

As the control group in non-acidic environment for expressing cp19k adhesive protein with the backbone of PSB1C3, on the direction for our target gene we use the constitutive promoter J23119 (BBa_J23119), RBS (BBa_B0034) is the ribosome binding site, the target gene - CsaA and mSandy2, Cp19k are connected with the sequence of GS linker, and the mSandy2 is the reporter gene and the double terminator (BBa_B0015) is the terminater we use in this circuit, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 2049
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2049
  Illegal NheI site found at 2085
  Illegal NheI site found at 2108
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2055
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2049
  Illegal XhoI site found at 1033
  Illegal XhoI site found at 1925
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 2049
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 2049
  Illegal XbaI site found at 2064
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  COMPATIBLE WITH RFC[1000]