Coding

Part:BBa_K4716001

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


cp19k (19 kDa cement protein)

The 19 kDa cement protein (cp19k), which is a self-assembly adhesion protein. It is extracted from barnacle and have an adhesion effect without the need of post-modification. For barnacles, the source of cp19k, their role is to help them anchor to the bottom of reefs and ships in order to maintain their position against ocean currents, which means cp19k is a very powerful bio-adhesive material in nature. In our project, because the L-DOPA group at the end of another adhesion protein mefp-5 may have the potential to disrupt the endocrine system in humans, we used Pasr promoter (BBa_K4716000) as the initiator of the adhesion module, and display it on E. coli DH5-alpha through CsgA fusion protein (BBa_K4716002), to test the adhesion properties of cp19k and to investigate whether it could be a better option for small intestinal surface adhesion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None