Part:BBa_K4711022

SpyTag

Usage and Biology

SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures.

Fig 1 SpyCatcher-SpyTag three-dimensional structure

References

[1]Keeble, Anthony H ,Banerjee,et al.Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.[J].Angewandte Chemie, 2017.DOI:10.1002/ange.201707623.

[2]Zakeri B , Fierer J O , Celik E ,et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proceedings of the National Academy of Sciences of the United States of America, 2012(12).DOI:10.1073/PNAS.1115485109. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]