Coding

Part:BBa_K4711013

Designed by: Qingyu Wu   Group: iGEM23_OUC-China   (2023-09-22)


Rubredoxin-NAD(+) reductase(alkT)

Usage and Biology

Involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.

Fig 1 The 3D structure of alkT

Source

Organism:Pseudomonas oleovorans

References

[1]Eggink G, Engel H, Vriend G, Terpstra P, Witholt B. Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J Mol Biol. 1990 Mar 5;212(1):135-42. doi: 10.1016/0022-2836(90)90310-I. PMID: 2319593. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None