Coding

Part:BBa_K4711011

Designed by: Qingyu Wu   Group: iGEM23_OUC-China   (2023-09-22)


Alkane 1-monooxygenase(alkB)

Usage and Biology

Catalyzes the hydroxylation of n-alkanes and fatty acids in the presence of a NADH-rubredoxin reductase and rubredoxin.

Fig 1 The 3D structure of alkB

Source

Organism:Pseudomonas oleovorans

References

[1] Kok M , Oldenhuis R , Linden M P G V D ,et al.The Pseudomonas oleovorans alkane hydroxylase gene[J]. 1989.DOI:10.1016/S0021-9258(18)83564-5. [2]Shanklin J, Whittle E. Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family. FEBS Lett. 2003 Jun 19;545(2-3):188-92. doi: 10.1016/s0014-5793(03)00529-5. PMID: 12804773.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 84
    Illegal NgoMIV site found at 996
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None