Coding

Part:BBa_K4710001

Designed by: Clara Deimling   Group: iGEM23_UChicago   (2023-10-09)


Aromatic Aminoadipate Transaminase (AATA)

This part codes for aromatic aminoadipate transaminase (AATA).

Usage and Biology

A type of transaminase: Pyridoxal phosphate (PLP)-dependent enzymes that catalyze the transfer of amino groups between amino acids and alpha-keto acids Derived from Saccharomyces cerevisiae

Expresses in BL21 cells grown in Terrific Broth to yield ~30 mg from a 1L pre-culture of TB inoculated with AATA-PQE81XN transformed BL21 cells with 1mM IPTG at 18C for 16 hours.

Binds with PLP to form a peak at 411 nm.

Reacts with L-phenylalanine as an amine donor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1012
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None