Composite

Part:BBa_K4706021

Designed by: Niklas K�ppers   Group: iGEM23_Duesseldorf   (2023-10-03)


SrpR SV40NLS for S. cerevisae expression

SrpR is a repressor usually only used in E. coli. This part is codon optimized for S. cerevisae expression to be used in repression systems in a S. cerevisae chassis. The fused SV40-NLS (BBa_K4706010) is necessary for a successful repression system for Chromosomal integrated DNA as its allows the SrpR to be localised inside the Nucleus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 244
    Illegal BglII site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


References: 1. Synthetic Toolkit for Complex Genetic Circuit Engineering in Saccharomyces cerevisiae; Anssi Rantasalo, Joosu ; Kuivanen, Merja Penttilä, Jussi Jäntti, and Dominik Mojzita; ACS Synthetic Biology 2018 7 (6), 1573-1587; DOI: 10.1021/acssynbio.8b00076

[edit]
Categories
//cds/transcriptionalregulator/repressor
//chassis/eukaryote/yeast
Parameters
directionforward