Plasmid

Part:BBa_K4706020

Designed by: Niklas Küppers   Group: iGEM23_Duesseldorf   (2023-10-01)


pSB1C30YIpHR-HO CUP1-GFP 2A SrpR-SV40NLS

pSB1C30YIpHR-HO CUP1-GFP 2A SrpR-SV40NLS

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal BglII site found at 3157
    Illegal BglII site found at 3877
    Illegal BglII site found at 4147
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 4123
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//dna/origin_of_replication
//primer/reporter/gfp
//ribosome/prokaryote/ecoli
Parameters
None