Part:BBa_K4705005
Temperature sensitive switch
Temperature sensitive switch Characterization In 2019, UCAS-China developed a collection of thermosensitive parts with high-performance, versatility and robustness. Based on TCI transcription factor family and TlpA family, they collected five TCI and TlpA mutants and used sfGFP as reporter to build some heat-inducible ON-switches, which can open gene expression under high temperature. To characterize them quantitatively, they first characterized the performance of them by flow cytometer in Top10 strain.
As shown in their study, most of the transcription repressors show sharp thermal transitions, especially TCI and TCI42, with more than 100-fold induction within 10 degrees Celsius. Their impressive performances make them candidate parts for our further circuit desig
So we choose this TCLprotein to be our temperature sensitive swith. With a promoter ,a RBS ,the coding sequence of TCL and a terminator we hope to use this protein to control the subsequent promoter in order to control the next target protein we need. Following information are writing in BBa K4705006 Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |