Part:BBa_K4701211

Glycolaldehyde dehydrogenase (aldA)

Glycolaldehyde dehydrogenase (UniProt: P25553) encoded by the aldA gene, is a native enzyme to E. coli that among others, catalyzes a reaction from glycolaldehyde to glycolate [1]. When overexpressed together with an engineered propanediol oxidoreductase (BBa_K4701210), E. coli can be engineered to utilize ethylene glycol as its carbon source [1], [2].

References

[1] Pandit, AV., et al (2021) Engineering Escherichia coli for the utilization of ethylene glycol. Microbial Cell Factories. 20(1), 22. https://doi.org/10.1186/s12934-021-01509-2

[2] Panda, S., et al (2021) Improving ethylene glycol utilization in Escherichia coli fermentation. Biochemical Engineering Journal. 168, 107957. https://doi.org/10.1016/j.bej.2021.107957

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]