Part:BBa_K4701210

Engineered L-1,2-propanediol oxidoreductase (fucO)

Propanediol oxidoreductase (UniProt: P0A9S1) encoded by the fucO gene, is a native enzyme to E. coli that has also been shown to exhibit promiscuous activity toward ethylene glycol [1]. This part contains a variant with two swapped residues I6L/L7V as they have been found to increase activity [2].

When overexpressed together with a glycolaldehyde dehydrogenase (BBa_K4701211), E. coli can be engineered to utilize ethylene glycol as its carbon source [2], [3].

See our wiki for more details on the design.

References

[1] Boronat, A., et al. (1983) Experimental evolution of a metabolic pathway for ethylene glycol utilization by Escherichia coli. Journal of Bacteriology. 153(1), 134–139. https://doi.org/10.1128/jb.153.1.134-139.1983

[2] Pandit, AV., et al (2021) Engineering Escherichia coli for the utilization of ethylene glycol. Microbial Cell Factories. 20(1), 22. https://doi.org/10.1186/s12934-021-01509-2

[3] Panda, S., et al (2021) Improving ethylene glycol utilization in Escherichia coli fermentation. Biochemical Engineering Journal. 168, 107957. https://doi.org/10.1016/j.bej.2021.107957

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 25