Coding

Part:BBa_K4701202

Designed by: Henri Sundquist   Group: iGEM23_Aalto-Helsinki   (2023-07-12)


FAST-PETase (Engineered PET hydrolase)

An improved version of IsPETase from I. sakaiensis (BBa_K1921000), with mutations N233K/R224Q/S121E/D186H/R280A [1]. The variant shows improved PET degradation activity over previous IsPETase and LCC variants at a wide range of temperatures from 30°C to 50°C. At sufficiently high concentrations FAST-PETase can fully degrade untreated consumer PET containers of moderate crystallinity.

This sequence was used in part BBa_K4701300, which is designed to produce extracellular FAST-PETase our of E. coli hosts.


References

[1] Lu, H., et al. (2022). Machine learning-aided engineering of hydrolases for PET depolymerization. Nature, 604(7907), 662–667. https://doi.org/10.1038/s41586-022-04599-z

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None