Tag

Part:BBa_K4701200

Designed by: Henri Sundquist   Group: iGEM23_Aalto-Helsinki   (2023-07-12)


pelB g58a signal peptide

The pelB signal peptide marks a protein for secretion out from an E. coli cell. This part differs from the standard pelB sequence (BBa_J32015) by a missense mutation caused by a substitution from guanine to adenine at position 58. This variant has been shown to exhibit improved secretory efficiency compared to the standard pelB sequence, when located upstream of IsPETase [1]. (Variant PM3 in the paper)


References

[1] Shi, L.,et al.. (2021). Enhanced extracellular production of IsPETase in Escherichia coli via engineering of the pelB signal peptide. Journal of Agricultural and Food Chemistry, 69(7), 2245–2252. https://doi.org/10.1021/acs.jafc.0c07469

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None