Part:BBa_K4695130

scfL-his

This is a protein-coding composite part consisting of fusion scaffoldin protein with a HisTag linker T5E_6H at the C-terminal end. This protein acts as a scafffolding, consisting of cellulose binding domain and three cohesin domains, that have strong affinity to corresponding dockerin domains.

Design Notes

This composite part consists of:

• ScfL coding sequence (BBa_K4695030)
• DT4_GS flexible linker (BBa_J435309)
• T4T5_GS flexible linker (BBa_J435349)
• C-terminal T5E_6H HisTag linker (BBa_J435369)

FLANKED 5' fusion site: AATG, 3' fusion site: GCTT.
Source sequences were optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

Source

ScfL:
Cellulose binding domain:
Organism: Acetovibrio thermocellus
Structure: https://www.uniprot.org/uniprotkb/Q06851/entry

Cohesin 1:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Cohesin 2:
Organism: Acetivibrio cellulyticus
Structure: https://www.rcsb.org/structure/4UYQ

Cohesin 3:
Organism: Bacteroides cellulosolvens
Structure: https://www.rcsb.org/structure/2Y3N

Registry of standard biological parts

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 589
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 768
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1659
  
• 1000
  COMPATIBLE WITH RFC[1000]