Part:BBa_K4695122
dcx3-docxylY
This is a protein-coding composite part consisting of two protein domain-coding sequences with the DT4_TEV linker between them and a HisTag linker T5E_6H at the C-terminal end. Expressing this sequence results in a fusion protein potentially capable of removing carboxylic group from phthalate mono-esters or phthalic acid. This protein can also bind to corresponding cohesin domain.
Design Notes
This composite part consists of:
- Dcx3 coding sequence (BBa_K4695022)
- DT4_TEV linker (BBa_J435395)
- DocXylY coding sequence (BBa_K4695040)
- C-terminal T5E_6H HisTag linker (BBa_J435369)
Sequence is designed in a way that allows user to swap enzymatic and dockerin domains with their counterparts.
FLANKED 5' fusion site: AATG, 3' fusion site: GCTT.
Source sequences were optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/
Source
Dcx3:
Organism: Pantoea dispersa BJQ0007
Article: https://doi.org/10.1016/j.jfca.2021.104201
DocXylY:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ
Registry of standard biological parts
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 170
Illegal PstI site found at 1114 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 170
Illegal PstI site found at 1114
Illegal NotI site found at 592 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 170
Illegal PstI site found at 1114 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 170
Illegal PstI site found at 1114 - 1000COMPATIBLE WITH RFC[1000]
None |