Part:BBa_K4695120

dcx1-docxylY

This is a protein-coding composite part consisting of two protein domain-coding sequences with the DT4_TEV linker between them and a HisTag linker T5E_6H at the C-terminal end. Expressing this sequence results in a fusion protein potentially capable of removing carboxylic group from phthalate mono-esters or phthalic acid. This protein can also bind to corresponding cohesin domain.

Design Notes

This composite part consists of:

• Dcx1 coding sequence (BBa_K4695020)
• DT4_TEV linker (BBa_J435395)
• DocXylY coding sequence (BBa_K4695040)
• C-terminal T5E_6H HisTag linker (BBa_J435369)

Sequence is designed in a way that allows user to swap enzymatic and dockerin domains with their counterparts.
FLANKED 5' fusion site: AATG, 3' fusion site: GCTT.
Source sequences were optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

Source

Dcx1:
Organism: Pantoea dispersa BJQ0007
Article: https://doi.org/10.1016/j.jfca.2021.104201

DocXylY:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Registry of standard biological parts

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 13
  Illegal EcoRI site found at 346
  Illegal EcoRI site found at 412
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 13
  Illegal EcoRI site found at 346
  Illegal EcoRI site found at 412
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 13
  Illegal EcoRI site found at 346
  Illegal EcoRI site found at 412
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 13
  Illegal EcoRI site found at 346
  Illegal EcoRI site found at 412
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 13
  Illegal EcoRI site found at 346
  Illegal EcoRI site found at 412
  Illegal AgeI site found at 223
  
• 1000
  COMPATIBLE WITH RFC[1000]