Part:BBa_K4695030

scfL

Coding sequence for fusion scaffoldin protein (ScfL). This protein acts as a scaffolding for proteins with corresponding dockerin domains. It consists of:

• Cellulose binding domain - allows strong biniding to cellulose
• Cohesins 1, 2 and 3 - allow binding of proteins with specific dockerin domains.

Design Notes

FLANKED 5' fusion site: AATG, 3' fusion site: AGGT.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has a START codon at the 5' end of the sequence and no STOP codon at the 3' end because it was designed as a part of a fusion protein. If you want to express only this coding sequence, please add the STOP codon in your design.

Source

Cellulose binding domain:
Organism: Acetovibrio thermocellus
Structure: https://www.uniprot.org/uniprotkb/Q06851/entry

Cohesin 1:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Cohesin 2:
Organism: Acetivibrio cellulyticus
Structure: https://www.rcsb.org/structure/4UYQ

Cohesin 3:
Organism: Bacteroides cellulosolvens
Structure: https://www.rcsb.org/structure/2Y3N

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 589
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 768
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1659
  
• 1000
  COMPATIBLE WITH RFC[1000]