Part:BBa_K4695010

pnbA

Coding sequence for para-nitrobenzyl esterase (PnbA). This enzyme (EC 3.1.1) from Bacillus subtilis BJQ0005 is capable of di-ester bond hydrolysis activity.

Design Notes

FLANKED 5' fusion site: AATG, 3' fusion site: AGGT.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has a START codon at the 5' end of the sequence and no STOP codon at the 3' end because it was designed as a part of a fusion protein with interchangable domains. If you want to express only this coding sequence, please add the STOP codon in your design.

Source

Organism: Bacillus Subtilis BJQ0005
GenBank page: https://www.ncbi.nlm.nih.gov/protein/QHH21706.1/
Article: https://doi.org/10.1016/j.envpol.2021.116461

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 534
  Illegal BamHI site found at 837
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 603
  
• 1000
  COMPATIBLE WITH RFC[1000]