Part:BBa_K4689112
Ketoisovalerate dehydrogenase (kivD)
KivD, also known as 3-ketoacyl-CoA thiolase, is a vital enzyme involved in the metabolism of fatty acids and amino acids. Specifically, it plays a central role in the biosynthesis of isovaleryl-CoA, a critical molecule in various metabolic pathways.
Role in the Isobutanol Pathway:
KivD is a key enzyme in the isobutanol pathway, where it catalyzes a critical step in the conversion of intermediates into isobutanol. This pathway often begins with the degradation of branched-chain amino acids, such as valine and leucine, to produce keto acids. These keto acids serve as precursors for isobutanol synthesis. KivD specifically acts on the keto acid precursor 2-ketoisovalerate (2-KIV), converting it into isobutyryl-CoA, an essential intermediate in the isobutanol pathway. This reaction is a pivotal step because isobutyryl-CoA is further processed by subsequent enzymes to produce isobutanol. The ability of KivD to efficiently convert 2-KIV into isobutyryl-CoA is central to the biosynthesis of isobutanol, making it a critical enzyme in the production of this biofuel and chemical compound.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
REFERENCES :
1.Blombach, B., & Eikmanns, B. J. (2011). Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum. Bioengineered bugs, 2(6), 346-350. 2.Smith, K. M., & Liao, J. C. (2011). An evolutionary strategy for isobutanol production strain development in Escherichia coli. Metabolic engineering, 13(6), 674-681.
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