Part:BBa_K4688007
FUCO3-GFP-expression
FUCO3 is one of the isoenzymes of Alpha-L-fucosidases. Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins and thus cleaving the nonreducing terminal-L-fucose from these glycoconjugates. L-fucose, a monosaccharide commonly found in various organisms serves as an important nutrient for the microorganism. It has been implicated in bacteria colonisation by acting as nutrient source for commensal or pathogenic bacteria . However, Qipengyuania aquimaris does not encode any obvious fucosidase homologs suggesting that Qipengyuania aquimaris may forage on L-fucose released by others. Alpha-L-fucosidases of Phaeodactylum tricornutum are predicted to be extracellular (membrane-attached or periplasm) or intracellular. Therefore, overexpression of alpha-L-fucosidases in Phaeodactylum tricornutum allows alpha-L-fucosidases to be released into the environment, where it can be utilizated by Qipengyuania aquimaris. The GFP gene was inserted downstream of the FUCO3 gene, thereby tagging FUCO3 to elucidate its expression or localization characteristics.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2347
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BamHI site found at 3498 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2347
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2347
Illegal NgoMIV site found at 2081 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 999
Illegal BsaI site found at 1202
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