Part:BBa_K4688006
FUCO2-GFP-expression
FUCO2 is one of the isoenzymes of Alpha-L-fucosidases. Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins and thus cleaving the nonreducing terminal-L-fucose from these glycoconjugates. L-fucose, a monosaccharide commonly found in various organisms serves as an important nutrient for the microorganism. It has been implicated in bacteria colonisation by acting as nutrient source for commensal or pathogenic bacteria . However, Qipengyuania aquimaris does not encode any obvious fucosidase homologs suggesting that Qipengyuania aquimaris may forage on L-fucose released by others. Alpha-L-fucosidases of Phaeodactylum tricornutum are predicted to be extracellular (membrane-attached or periplasm) or intracellular. Therefore, overexpression of alpha-L-fucosidases in Phaeodactylum tricornutum allows alpha-L-fucosidases to be released into the environment, where it can be utilizated by Qipengyuania aquimaris. The GFP gene was inserted downstream of the FUCO2 gene, thereby tagging FUCO2 to elucidate its expression or localization characteristics.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1706
Illegal SpeI site found at 1936 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1706
Illegal SpeI site found at 1936 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1706
Illegal BglII site found at 1
Illegal BamHI site found at 3438 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1706
Illegal SpeI site found at 1936 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1706
Illegal SpeI site found at 1936
Illegal NgoMIV site found at 1788 - 1000COMPATIBLE WITH RFC[1000]
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