Coding

Part:BBa_K4688004

Designed by: Xiaoxin Xuan   Group: iGEM23_FZPt-CHINA   (2023-09-30)


FUCO3

FUCO3 is one of the isoenzymes of Alpha-L-fucosidases. Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins and thus cleaving the nonreducing terminal-L-fucose from these glycoconjugates. L-fucose, a monosaccharide commonly found in various organisms serves as an important nutrient for the microorganism. It has been implicated in bacteria colonisation by acting as nutrient source for commensal or pathogenic bacteria . However, Qipengyuania aquimaris does not encode any obvious fucosidase homologs suggesting that Qipengyuania aquimaris may forage on L-fucose released by others. Alpha-L-fucosidases of Phaeodactylum tricornutum are predicted to be extracellular (membrane-attached or periplasm) or intracellular. Therefore, overexpression of alpha-L-fucosidases in Phaeodactylum tricornutum allows alpha-L-fucosidases to be released into the environment, where it can be utilizated by Qipengyuania aquimaris.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1407
    Illegal PstI site found at 1139
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1139
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1407
    Illegal PstI site found at 1139
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1407
    Illegal PstI site found at 1139
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 59
    Illegal BsaI site found at 262


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Categories
Parameters
None