Composite

Part:BBa_K4687029

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-07)


MAD7+recE/T+pBluescript+PlacM:MADE/TBlacM

The exonuclease–recombinase pair recE/T can improve dsDNA recombination efficiency in C. glutamicum.In this study, we combined the recE/T and CRISPR-MAD7 systems.Using this system, we optimized the CRISPR-MAD7 promoter sequence.And we will change the plasmid vector to obtain the recombinant plasmid, select the best plasmid vector, and improve the editing efficiency of the target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4571
    Illegal XbaI site found at 4541
    Illegal SpeI site found at 4547
    Illegal PstI site found at 4565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4571
    Illegal SpeI site found at 4547
    Illegal PstI site found at 4565
    Illegal NotI site found at 4533
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4571
    Illegal BglII site found at 714
    Illegal BglII site found at 762
    Illegal BglII site found at 1083
    Illegal BglII site found at 2298
    Illegal BglII site found at 2950
    Illegal BglII site found at 3804
    Illegal BamHI site found at 4553
    Illegal XhoI site found at 4604
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4571
    Illegal XbaI site found at 4541
    Illegal SpeI site found at 4547
    Illegal PstI site found at 4565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4571
    Illegal XbaI site found at 4541
    Illegal SpeI site found at 4547
    Illegal PstI site found at 4565
    Illegal NgoMIV site found at 4192
    Illegal AgeI site found at 1865
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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