DNA

Part:BBa_K4687020

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


Donor:crtI

CRISPR-MAD7 nuclease binds to the downstream of the PAM region of the target DNA under the guidance of sgRNA. The active region of the protein will cut out a sticky end of the target DNA, and then introduce a DNA fragment homologous to the end (donor DNA) and the broken DNA for homologous recombination repair. In this project, we designed donor DNA to interact with CRISPR-MAD7 gene editing systems to achieve targeted gene knockout.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1005
    Illegal PstI site found at 483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1005
    Illegal NheI site found at 870
    Illegal PstI site found at 483
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1005
    Illegal BglII site found at 1248
    Illegal BglII site found at 1611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1005
    Illegal PstI site found at 483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1005
    Illegal PstI site found at 483
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None