Plasmid

Part:BBa_K4687008

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pXMJ19

The pXMJ19 vector is a self-replicating DNA molecule that transfers DNA fragments (target genes) to a recipient cell under a genetically engineered recombinant DNA technology. The pXMJ19 vector in this experiment introduced CRISPR components into cells, and editing of target genes was performed by expression of Cas protein and gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5749
    Illegal XbaI site found at 5722
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5749
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5749
    Illegal BamHI site found at 5728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5749
    Illegal XbaI site found at 5722
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5749
    Illegal XbaI site found at 5722
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
    Illegal NgoMIV site found at 3638
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None