Plasmid

Part:BBa_K4687007

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pBluescript

The pBluescript vector is a self-replicating DNA molecule that transfers DNA fragments (target genes) to a recipient cell under a genetically engineered recombinant DNA technology. The pBluescript vector in this experiment introduced CRISPR components into cells, and editing of target genes was performed by expression of Cas protein and gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 707
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 707
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
    Illegal NotI site found at 669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 707
    Illegal BamHI site found at 689
    Illegal XhoI site found at 740
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 707
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 707
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
    Illegal NgoMIV site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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