Plasmid

Part:BBa_K4687006

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pEC-XK99E

The pEC-XK99E vector is a self-replicating DNA molecule that transfers DNA fragments (target genes) to a recipient cell under a genetically engineered recombinant DNA technology. The pEC-XK 99 E vector in this experiment introduced CRISPR components into cells, and editing of target genes was performed by expression of Cas protein and gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 92
    Illegal NheI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1382
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 2046
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1895
    Illegal SapI.rc site found at 2105
    Illegal SapI.rc site found at 3078


[edit]
Categories
Parameters
None