Regulatory

Part:BBa_K4687005

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-07)


The promoter Ptuf

We ligated the designed Ptuf promoter sequence with CRISPR-MAD7 nuclease to construct an expression vector. The expression vector was transformed into E. coli to amplify the plasmid and verified to be correct by sequencing, and then transformed into Corynebacterium glutamicum as a way to observe the expression effect of the CRISPR-MAD7 system. It was concluded whether the Ptuf promoter could enhance the expression of CRISPR-MAD7 system in Corynebacterium glutamicum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None