Regulatory

Part:BBa_K4687002

Designed by: Tianyi Liang   Group: iGEM23_HBUT-China   (2023-09-30)


The promoter PlacM

The designed PlacM promoter sequence was ligated with CRISPR-MAD7 nuclease to construct an expression vector. The expression vector was transformed into E. coli to amplify the plasmid and verified to be correct by sequencing, and then transformed into Corynebacterium glutamicum as a means of observing the expression effect of the CRISPR-MAD7 system. It was observed whether the PlacM promoter could enhance the expression of CRISPR-MAD7 system in Corynebacterium glutamicum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None