Part:BBa_K4685014
Polyethylene specific peptide sequence
This basic part code for a peptide capable of attaching the polyethylene. This part is a subunit of the CsgA-peptide complex(BBa_K4685042).
To increase the curli fiber capacity to capture microplastic, we planned to fuse these peptide sequences to the CsgA( main subunit of the curli fiber) gene via a short flexible linker.
The peptides are short enough that they do not disturb the proper folding of the curli fibers. The idea of this design is that the peptide stretches out of the beta-sheet structure of the curli fibers and has the possibility to interact with the external environment
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
iGEM Team REC-CHENNAI 2024 (Contribution)
Group: TEAM REC-CHENNAI 2024. Authors: Maline.M, Rhea Harry William. We came across the iGEM team UNILausanne 2023 - Plastic Busters, whose project closely aligns with ours. We have tried to add another layer to their work on polyethylene specific peptide sequence (BBa_K4685014). We have developed polyethylene microplastic containing 18 chains and chain length 160 monomers which is energy minimised. The interaction of this microplastic with the microplastic binding peptide represented by the part BBa_K4685014 was further simulated in GROMACS, enhancing understanding and approach to the project.
Fig.1: Energy minimised coarse grained peptides and polymer within a simulation box.
Fig.2: MD simulated coarse grain peptide - polymer model
Fig.3: Analysis of interactions between peptide - polymer model
References
Vismara A, Gautieri A. Molecular insights into nanoplastics-peptides binding and their interactions with the lipid membrane. Biophys Chem. 2024 May;308:107213. doi: 10.1016/j.bpc.2024.107213.
Gabbrielli S, Colnaghi L, Mazzuoli-Weber G, Redaelli ACL, Gautieri A. In Silico Analysis of Nanoplastics' and β-amyloid Fibrils' Interactions. Molecules. 2023 Jan 2;28(1):388. doi: 10.3390/molecules28010388.
Panizon E, Bochicchio D, Monticelli L, Rossi G. MARTINI Coarse-Grained Models of Polyethylene and Polypropylene. J Phys Chem B. 2015 Jun 25;119(25):8209-16. doi: 10.1021/acs.jpcb.5b03611.
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