Part:BBa_K4680008

FMO3 Homo S. Generator

Usage and Biology

The FSU iGEM team created this biological device to oxide trimethylamine (TMA) in the small intestine of a human. The purpose behind this device is to give back the FMO3 enzyme function to those who have little to no activity in their body. Those people suffer from a disease called Trimethylaminuria, or TMAU. TMAU is a rare disease in which a mutation appears on the FMO3 enzyme coding sequence and the body can no longer oxidize trimethylamine into trimethylamine N-oxide in the liver. This causes the person to expel an unpleasant odor throughout their entire body. Our device consists of an Anderson promoter, synthetic RBS, a FMO3 coding sequence, and a terminator. The Flavin containing diananiline monooxygenase 3 coding sequence comes from the human genome.

Measurments

This biological device has been inserted in a pIDTSmart-Kan plasmid backbone from IDT resulting in pFSUIGEM202. Figure 1 depicts the map of pFSUIGEM202.

Figure 1 - pFSUIGEM202

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1220
  Illegal PstI site found at 1583
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 1220
  Illegal PstI site found at 1583
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1220
  Illegal PstI site found at 1583
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1220
  Illegal PstI site found at 1583
  Illegal NgoMIV site found at 934
  Illegal AgeI site found at 434
  Illegal AgeI site found at 913
  Illegal AgeI site found at 1055
  
• 1000
  COMPATIBLE WITH RFC[1000]