Part:BBa_K4674003
Single-strand binding protein (SSBP)
ssDNA binding proteins: the EcSBP
Single-stranded DNA binding protein (SBP) sequester and protect the ssDNA. In E. Coli, the SBP (EcSBP; Genebank: QGY26441.1) is associated with the genome stability. The N-terminal of EcSBP is responsible for the ssDNA binding.
Fig. The structure of tetrameric SBPs from Shishmarev et. al. 2014
Experimental result
After cloning into the pET15b vector, We induced the RepA and SSBP protein expression by IPTG. After 6 hours of IPTG induction at 37°C, we found that the RepA and SSBP were expressed. However, the expression of protein was found in the inclusion body (cell pellet).
Fig. The coomassie blue staining of SDS-PAGE analysis of RepA and SSBP protein induction. S: supernatant; P: cell pellet.
References Shishmarev D, et al. (2014) Intramolecular binding mode of the C-terminus of Escherichia coli single-stranded DNA binding protein determined by nuclear magnetic resonance spectroscopy Nucleic Acids Res. 42(4):2750-7.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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